{"id":12736,"date":"2020-06-11T16:34:46","date_gmt":"2020-06-11T11:04:46","guid":{"rendered":"http:\/\/udaen.info\/?p=12736"},"modified":"2020-06-11T16:34:46","modified_gmt":"2020-06-11T11:04:46","slug":"new-low-cost-novel-coronavirus-test-developed","status":"publish","type":"post","link":"https:\/\/udaen.info\/?p=12736","title":{"rendered":"New low-cost novel coronavirus test developed"},"content":{"rendered":"<div class=\"MinistryNameSubhead text-center\">Ministry of Science &amp; Technology<\/div>\n<div class=\"text-center\">\n<h2>New low-cost novel coronavirus test developed<br \/>\n<span id=\"ltrSubtitle\"><\/span><\/h2>\n<\/div>\n<div class=\"ReleaseDateSubHeaddateTime text-center pt20\">\u00a0PIB Delhi<\/div>\n<div class=\"pt20\"><\/div>\n<p>The Indian Council of Medical Research (ICMR) has recommended only reverse transcription polymerase chain reaction (RT-qPCR) test for novel coronavirus testing. Researchers at the Centre for Cellular and Molecular Biology (CCMB) have developed a new low-cost and low-tech required test for SARS-CoV-2 testing. This test is known as reverse transcription nested PCR (RT-nPCR) test.<\/p>\n<p>This test does not require real-time quantitative RT-qPCR. The RT-nPCR developed by the CCMB research team has shown\u00a0 comparable performance to the standard RT-qPCR test. The nested PCR (RT-nPCR) approach does not depend on RT-qPCR but uses standard RT-PCR as part of an endpoint assay.<\/p>\n<p><img decoding=\"async\" src=\"http:\/\/164.100.117.97\/WriteReadData\/userfiles\/image\/image003M5DN.jpg\" \/><\/p>\n<p>In the course of comparing the results of both tests, researchers found that the standard RT-qPCR test can have low detection efficiency (less than 50%) in a real testing scenario, which may be due to low viral representation in many samples. This finding brought home the importance of monitoring detection efficiency directly in test environments.<\/p>\n<p>\u201cWe developed and tested an RT-nPCR protocol comprising a multiplex primary RT-PCR for amplification of four SARS-CoV-2 amplicons and a control human amplicon followed by a secondary nested PCR for individual amplicons. We also examined the use of RT-nPCR in pooled testing and in direct amplification without RNA isolation,\u201d said Dr Rakesh Mishra, Director, CCMB, while speaking with\u00a0<em>India Science Wire<\/em>.<\/p>\n<p>The RNA isolated from nasopharyngeal<strong>\u00a0<\/strong>swab samples that had been previously tested using one of the two RT-qPCR tests was examined using RT-nPCR and the results were compared. It was found that taking both standard RT-qPCR tests together, the RT-nPCR test was able to identify 90% of the detected samples as positive by RT-qPCR. It also detected 13% samples as positive among samples that were negative by the standard RT-qPCR test (likely false negatives). Based on the experimentally measured false negative rate by RT-nPCR tests from this study, it was estimated that as many as 50% of positive samples may escape detection in single pass testing by RT-qPCR in an actual testing scenario.<\/p>\n<p>\u201cThis new test is awaiting approval from ICMR. We might ask ICMR to use this test in those places where there are no RT-qPCR machines,\u201d remarked Dr Mishra.<\/p>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Ministry of Science &amp; Technology New low-cost novel coronavirus test developed \u00a0PIB Delhi The Indian Council of Medical Research (ICMR) has recommended only reverse transcription polymerase chain reaction (RT-qPCR) test for novel coronavirus testing. Researchers at the Centre for Cellular and Molecular Biology (CCMB) have developed a new low-cost and low-tech required test for SARS-CoV-2 [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"om_disable_all_campaigns":false,"_exactmetrics_skip_tracking":false,"_exactmetrics_sitenote_active":false,"_exactmetrics_sitenote_note":"","_exactmetrics_sitenote_category":0,"_monsterinsights_skip_tracking":false,"_uf_show_specific_survey":0,"_uf_disable_surveys":false,"footnotes":""},"categories":[74,12],"tags":[],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/udaen.info\/index.php?rest_route=\/wp\/v2\/posts\/12736"}],"collection":[{"href":"https:\/\/udaen.info\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/udaen.info\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/udaen.info\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/udaen.info\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=12736"}],"version-history":[{"count":1,"href":"https:\/\/udaen.info\/index.php?rest_route=\/wp\/v2\/posts\/12736\/revisions"}],"predecessor-version":[{"id":12737,"href":"https:\/\/udaen.info\/index.php?rest_route=\/wp\/v2\/posts\/12736\/revisions\/12737"}],"wp:attachment":[{"href":"https:\/\/udaen.info\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=12736"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/udaen.info\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=12736"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/udaen.info\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=12736"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}